Published 2020
| Version v1
Dataset
Open
non_treated_scale8
Description
Raw data files of non_treated_scale8
Files
keyimg_wnj2020-02-12-15.jpg
Technical info
Tilt Series Date: 2020-02-12
Data Taken By: William Nicolas Species / Specimen: Allium cepa Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 80.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x. Microscope: Caltech Titan Krios Acquisition Software: Serial EM Upload Method: webload Processing Software Used: IMOD Purification / Growth Conditions / Treatment: Onion peels generated like Kafle et al. 2013, incubated in HEPES >20min until freezing Sample Preparation: Glow-discharged (15mA – 1min) Quantifoil R2/2 NH2 Cu EM grids (EMSdiasum). Plunge freezing was performed with a 60/40 ratio ethane/propane mix and an FEI Vitrobot Mark IV (Thermo Fisher). Humidity was set at 50%, temperature at 20°C. Grids were first manually backblotted for 6 s in order to attach the cell wall peel firmly to the carbon, followed by two autoblottings (front and back) 5 s, maximal blot force (25) and a drain time of 3 s.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-02-12-15/rawdata/
on17-1_lam3_ts2.st, Tilt Series (Pixel Size 0.32 nm), 1.6 GB Download on17-1_lam3_ts2.rec, Reconstruction (Pixel Size 1.32 nm), 628.2 MB Download on17-1_lam3_ts2.rawtlt, 272.0 B Download on17-1_lam3_ts2.mrc.mdoc, 19.2 kB DownloadAdditional details
- DOI
- 10.22002/9540f-kzj58
- TILT_SERIES_ID
- wnj2020-02-12-15
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2020-02-12
- Created
-
2022-03-29
- Updated
-
2022-03-29