Published 2015 | Version v1
Dataset Open

Dflg

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is a flagellum mutant from Sahand (ATP connected) grown in serum bottle. We saw with perfusion setup that the same mutant makes nanowires. So I went back and imaged the serum bottle version just to make sure that it doesnt have nanowires.

Technical info

Tilt Series Date: 2015-08-20

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand

Sample Preparation: same as before

Attached Files

dflgwithnanowires_atlas10029_part61_2.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB

dflgwithnanowires_atlas10029.mrc, Tilt Series (Pixel Size 0.6 nm), 1.7 GB

Files

keyimg_psa2015-08-20-29.jpg

Files (2.2 GB)

Name Size
1.7 GB (external) Download
445.4 MB (external) Download
md5:81a013c1af7622579a5a7589e76990d7
1.0 MB Preview Download
md5:8004d9831114e3e49301581bd9d23679
15.7 MB Preview Download
md5:c8689339cae3d9c9b712d6aaee51beb4
421.1 kB Preview Download
md5:9979e65bae177a2ee049be332498da7e
385.7 kB Preview Download

Additional details

Identifiers

DOI
10.22002/97hfw-xct65
TILT_SERIES_ID
psa2015-08-20-29

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-08-20
Created
2015-08-28
Updated
2016-09-07