Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

V.cholerae

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.

Technical info

Tilt Series Date: 2017-01-06

Data Taken By: Poorna Subramanian

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Yi-wei

Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.

psa2017-01-06-101_slicer17544.jpg: filament? psa2017-01-06-101_slicer17545.jpg: filament? psa2017-01-06-101_slicer17546.jpg: filament? psa2017-01-06-101_slicer17547.jpg: vesicle coated with something? psa2017-01-06-101_slicer17548.jpg: DNA? psa2017-01-06-101_slicer17549.jpg: DNA leaking psa2017-01-06-101_slicer17550.jpg: DNA psa2017-01-06-101_slicer17551.jpg: filament? psa2017-01-06-101_slicer17552.jpg: DNA? psa2017-01-06-101_slicer17553.jpg: DNA an filaments interwined with DNA? psa2017-01-06-101_slicer17554.jpg: coated vesicles psa2017-01-06-101_slicer17555.jpg: coated vesicles psa2017-01-06-101_slicer17556.jpg: phage?vesicle?

Attached Files

sq110009_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB

sq110009.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Files

keyimg_psa2017-01-06-101.jpg

Files (4.2 GB)

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3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/98hty-ncj32
TILT_SERIES_ID
psa2017-01-06-101

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-01-06
Created
2017-01-10
Updated
2017-01-24