Caltech Tomography Archive
Published 2014 | Version v1
Dataset Open

Hippocampal Neurons (cultured)

Swulius, Matt1
  • 1. ROR icon California Institute of Technology
Researchers:
Zhuang, Xiawei
Zhou, Ruobo

Description

Plunge frozen at DIV 14

Files

keyimg_mst2014-10-18-29.jpg
Files (22.2 MB)
Name Size
keymov_mst2014-10-18-29.mp4
md5:d0bc497c8a7beac1b4827ff854966b04
21.1 MB Download
keyimg_mst2014-10-18-29.jpg
md5:5c765358ced780b76eded86f39752b4f
1.1 MB Preview Download

Technical info

Tilt Series Date: 2014-10-18

Data Taken By: Matt Swulius

Species / Specimen: Rat Hippocampal Neuron (cultured)

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)

Purification / Growth Conditions / Treatment: Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.

Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.

External Files

Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-10-18-29/rawdata/

101814_2_hipp_neuron_27kx0001.mrc, Tilt Series (Pixel Size 0.41 nm), 3.4 GB Download

101814_2_hipp_neuron_27kx0001_full.rec, Reconstruction (Pixel Size 1.64 nm), 534.5 MB Download

Additional details

Identifiers Funding Dates
Created:
May 23, 2023
Modified:
August 27, 2025