Published 2013 | Version v1
Dataset Open

Agro Bald VirB8-GFP

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

the bald strain lacks flagellin 1-3 and is considered non-motile

Technical info

Tilt Series Date: 2013-07-02

Data Taken By: Elitza Tocheva

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Pat Zambryski

Purification / Growth Conditions / Treatment: cells were grown at 19C and induced for T4SS

Sample Preparation: Vitrobot

Attached Files

bald_gfp20007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

bald_gfp20007_part121_3.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_etl2013-07-02-6.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:9b4e3b333d16ae6e8b2264349bd59a4e
1.1 MB Preview Download
md5:be43c94dc231696f394a53b8c7e76416
20.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/9cvrz-r1s44
TILT_SERIES_ID
etl2013-07-02-6

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-07-02
Created
2013-07-02
Updated
2015-01-05