Published 2011 | Version v1
Dataset Open

TRIM5-21R 2D lattice

  • 1. ROR icon California Institute of Technology

Description

TRIM5-21R 2D lattice prepared by Barbie Ganser-Pornillos and Viswanathan Chandrasekaran

Technical info

Tilt Series Date: 2011-11-19

Data Taken By: Cora Woodward

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward and Kelly Dryden

Sample Preparation: manual blotting

Attached Files

12nov19_TRIM5_21R_grid2_AT2BT1_0004.mrc, Tilt Series (Pixel Size 0.45 nm), 3.4 GB

12nov19_TRIM5_21R_grid2_AT2BT1_0004_full.rec, Reconstruction (Pixel Size 1.8 nm), 530.0 MB

Files

keyimg_cwl2011-11-19-2.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
530.0 MB (external) Download
md5:810c0daafcf8cc6a6fde329a8c8d57c9
1.1 MB Preview Download
md5:b5a999924ec2f3ad58c8b5cd4db12456
20.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/9dzbw-wwr05
TILT_SERIES_ID
cwl2011-11-19-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-11-19
Created
2012-11-20
Updated
2019-03-07