U2OS-Tubulin Tracker Green
Description
Technical info
Tilt Series Date: 2017-07-15
Data Taken By: Wei Zhao
Species / Specimen: U2OS
Strain:
Tilt Series Settings: Single Axis, tilt range: (-62.0°, 62.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD-patch tracking
Purification / Growth Conditions / Treatment: U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.
Sample Preparation: Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).
Attached Files
G7_TS4.mrc, Tilt Series (Pixel Size 0.4569 nm), 1.7 GB
G7_TS4_bin2.rec, Reconstruction (Pixel Size 0.0 nm), 147.0 MB
Files
keyimg_wza2017-07-15-5.jpg
Additional details
Identifiers
- DOI
- 10.22002/9h88g-t3c85
- TILT_SERIES_ID
- wza2017-07-15-5
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-07-15
- Created
-
2017-07-17
- Updated
-
2017-09-14