Myxococcus xanthus PilO-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-10
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-10-19_slicer3736.jpg: Extended T6SS sheath in Myxococcus xanthus ycw2014-03-10-19_slicer7257.jpg: filaments
Attached Files
20140310_YWC092_10019.mrc, Tilt Series (Pixel Size 3.9 nm), 3.7 GB
20140310_YWC092_10019_part131_8.rec, Reconstruction (Pixel Size 15.6 nm), 712.7 MB
Files
keyimg_ycw2014-03-10-19.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:ae4c4635a26a85fa32cbd86661a8cebb
|
1.0 MB | Preview Download |
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md5:e93c03c1d6750c93c29121ac6a83f757
|
22.7 MB | Preview Download |
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md5:0222f8a4321457ef86e434a267c2a2d8
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384.6 kB | Preview Download |
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md5:fe66808a1ef6080708615601a4ec3cd9
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355.6 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/9jg4b-bbh28
- TILT_SERIES_ID
- ycw2014-03-10-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-10
- Created
-
2014-03-11
- Updated
-
2014-07-17