CLEM of TRIM5a-YFP in HeLa cells
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-01-22
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Tom Hope
Purification / Growth Conditions / Treatment: HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.
Attached Files
tomgraphytest17.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
tomgraphytest17_full.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB
Files
keyimg_scd2015-01-22-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/9kmrk-sy640
- TILT_SERIES_ID
- scd2015-01-22-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-01-22
- Created
-
2015-01-22
- Updated
-
2015-05-07