Myxococcus xanthus pilM-YFP

Description

Technical info

Tilt Series Date: 2014-05-16

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

ycw2014-05-16-10_slicer6867.jpg: Nested vesicle ycw2014-05-16-10_slicer6868.jpg: External vesicle ycw2014-05-16-10_slicer6870.jpg: Nested vesicle ycw2014-05-16-10_slicer6871.jpg: Peptidoglycan ycw2014-05-16-10_slicer6872.jpg: filament ycw2014-05-16-10_slicer6873.jpg: tube

Attached Files

20140516_Janelia_YWC096_10005_full.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB

20140516_Janelia_YWC096_10005.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB