V. cholerae RT4236 (stationary)
Description
Technical info
Tilt Series Date: 2015-08-10
Data Taken By: Yi-Wei Chang
Species / Specimen: Vibrio cholerae
Strain: O395-N1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: reupload_downloaded
Processing Software Used: SIRT
Collaborators and Roles: Strain from Ronald K. Taylor
Purification / Growth Conditions / Treatment: Overnight cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Take the sedimented cell clumps.
Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20150810_AK20_20055_SIRT_2K.rec, Reconstruction (Pixel Size 0.0 nm), 14.2 GB
20150810_AK20_20055.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
Files
keyimg_ycw2015-08-10-55.jpg
Additional details
Identifiers
- DOI
- 10.22002/9q7rj-3qh29
- TILT_SERIES_ID
- ycw2015-08-10-55
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-08-10
- Created
-
2015-08-11
- Updated
-
2015-09-09