lighlyfixed_wt_serumbottle
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-07-06
Data Taken By: Poorna Subramanian
Species / Specimen: Shewanella oneidensis
Strain: MR-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Sahand and Moh USC
Purification / Growth Conditions / Treatment: Grown using serum bottle. This sample was lightly fixed with 2.5% glutaraldehyde, the very same sample that was sent for SEM. After mixing with glutaraldehyde in a tube, the sample was applied on grid and frozen.
Sample Preparation: blotting conditions: 4 ul sample, blot force 6, blot time 3, drain time 1s
Attached Files
atlas1_fixedwt_serumbottle0004.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
atlas1_fixedwt_serumbottle0004_part121_57.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
Files
keyimg_psa2015-07-06-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/9v8tc-4tv60
- TILT_SERIES_ID
- psa2015-07-06-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-07-06
- Created
-
2015-07-07
- Updated
-
2015-07-08