Published 2015 | Version v1
Dataset Open

wt_serum_blotfrombehind

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is a sample of wt grown in serum bottle that made nanowires.

Technical info

Tilt Series Date: 2015-07-26

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand

Sample Preparation: The sample was put on the C side (glow discharged side) on the left. The blotting was done from behind on the rt side. Vitrobot was used. All other vitrobot conditions are the same.

psa2015-07-26-65_slicer17210.jpg: filament inside cell with head

Attached Files

10066.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB

10066_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB

Files

keyimg_psa2015-07-26-65.jpg

Files (2.2 GB)

Name Size
1.7 GB (external) Download
445.4 MB (external) Download
md5:fdac396cd6edc5e187e22900a41dac88
1.0 MB Preview Download
md5:1ce45ad4284cf180eaafee60a838a12e
15.4 MB Preview Download
md5:b6304e2f9e6a65dd1ca002f3ba309d94
392.7 kB Preview Download
md5:04b59595390246623a4d5f85ae2acc35
586.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/a0jea-egc88
TILT_SERIES_ID
psa2015-07-26-65

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-07-26
Created
2015-07-26
Updated
2017-01-02