V. cholerae RT4236 (refreshed)

Description

Technical info

Tilt Series Date: 2015-04-08

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: O395-N1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: SIRT

Collaborators and Roles: Strain from Ronald K. Taylor.

Purification / Growth Conditions / Treatment: ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin was spun down and resuspended in fresh LB with IPTG/ampicilin and grown for 3 hours. The cells was idle for 30 min at RT during grid preperations prior to freezing.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

ycw2015-04-08-7_slicer16533.jpg: TCP bundle

Attached Files

20150408_AK004_10007_SIRT_2k.rec, Reconstruction (Pixel Size 0.0 nm), 5.7 GB

20150408_AK004_10007.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB