Caltech Tomography Archive
Published 2011 | Version v1
Dataset Open

E. coli (pLE7, 20 uM IPTG)

Swulius, Matt1
  • 1. ROR icon California Institute of Technology

Description

Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.

Files

mst2011-06-02-8_slicer9688.jpg
Files (27.4 MB)
Name Size
mst2011-06-02-8_slicer9688.jpg
md5:53337bd4e12dbbed48cba25223dd56d3
325.7 kB Preview Download
keymov_mst2011-06-02-8.mp4
md5:c496888b1e14d06b3d67ae3b90196436
24.7 MB Download
keyimg_mst2011-06-02-8.jpg
md5:32e8cdc9a99e52102953c21ee56367cf
1.2 MB Preview Download
mst2011-06-02-8_slicer9683.jpg
md5:25fcf57d719a654cc08da9f86d329fb1
357.3 kB Preview Download
mst2011-06-02-8_slicer9684.jpg
md5:b811e2a5554bfd8516dedfe558f3f257
429.2 kB Preview Download
mst2011-06-02-8_slicer9685.jpg
md5:5b95390791e7ca5845b41b958b5f187a
437.1 kB Preview Download

Technical info

Tilt Series Date: 2011-06-02

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Gift from Larry Rothfield

Purification / Growth Conditions / Treatment: Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.

Sample Preparation: Vitrobot plunge freezing

mst2011-06-02-8_slicer9683.jpg: Escherichia coli . vesicle mst2011-06-02-8_slicer9684.jpg: Escherichia coli . vesicle mst2011-06-02-8_slicer9685.jpg: Escherichia coli . filament bundle . contains MreB mst2011-06-02-8_slicer9688.jpg: Escherichia coli . vesicle

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-8/rawdata/

20110602_PLE7_13.mrc, Tilt Series (Pixel Size 0.95 nm), 1.0 GB Download

20110602_PLE7_13_full.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB Download

Additional details

Identifiers Funding Dates
Created:
May 17, 2023
Modified:
August 29, 2025