wt_perfusion_fixed

Description

Technical info

Tilt Series Date: 2015-09-19

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 135.0 eV/Ų, defocus: -10.0 μm, magnification: 41000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: The cells look good on scope. Next time use 1.5ul gold.

Collaborators and Roles: Moh and Sahand USC

Purification / Growth Conditions / Treatment: This sample is wt cells grown in perfusion set-up. It is fixed with 2.5% glutaraldehyde for 15 minutes. The grid was prepared by Sahand on 9/10/15. The grid was triple rinsed in distilled water and saved in distilled water until freezing on 9/15/15 at USC using manual plunger.

Sample Preparation: This is gold grid with honey C. The grid was pulled out quickly from media. 1.2ul of 10nm gold was added immediately to the cells on the C side -same side as cells were present. The grid was loaded on manual plunger and blotted from behind- opposite of C side. And then plunge frozen.

Attached Files

091915_perfusionwtfixed_sq70009_part121_29.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB

091915_perfusionwtfixed_sq70009.mrc, Tilt Series (Pixel Size 0.26 nm), 3.4 GB