Myxococcus xanthus PilP-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-08-12_slicer3741.jpg: Myxococcus pilP-sfGFP. . . Double T6SS . . LR
Attached Files
20140308_YWC093_10033.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
20140308_YWC093_10033_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2014-03-08-12.jpg
Additional details
Identifiers
- DOI
- 10.22002/a9mjr-st388
- TILT_SERIES_ID
- ycw2014-03-08-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-08
- Created
-
2014-03-11
- Updated
-
2014-03-17