Published 2016 | Version v1
Dataset Open

Planktonic cell of Proteus

Creators

  • 1. ROR icon California Institute of Technology

Description

Planktonic cell, PG layer, Proteus mirabillis

Technical info

Tilt Series Date: 2016-06-11

Data Taken By: Qing Yao

Species / Specimen: Proteus mirabilis

Strain: HI4320

Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFtomo

Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university

Purification / Growth Conditions / Treatment: OD=6, 4 ul loading. blotting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 195/A2. defocus: -10um. magnification: 22500.

Attached Files

square30007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.2 GB

square30007_part111_3.rec, Reconstruction (Pixel Size 1.92 nm), 311.8 MB

Files

keyimg_qya2016-06-11-10.jpg

Files (3.5 GB)

Name Size
md5:59a9e7582dc10cbd4b72f6edcd323440
1.1 MB Preview Download
md5:f12c3f6836838606bf0f25e887745250
12.0 MB Preview Download
3.2 GB (external) Download
311.8 MB (external) Download

Additional details

Identifiers

DOI
10.22002/aahfb-th907
TILT_SERIES_ID
qya2016-06-11-10

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-06-11
Created
2016-06-20
Updated
2022-10-12