Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Agro_AD1408_GFP-VirB11

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

AD1408: pAdi16/A348dB11; Kanamycin 50; Functional GFP-VirB11 fusion.

Files

keyimg_dga2015-07-21-26.jpg
Files (14.1 MB)
Name Size
keyimg_dga2015-07-21-26.jpg
md5:d89118d0124a77c8b7b7ca4c5ae8d306
1.0 MB Preview Download
keymov_dga2015-07-21-26.mp4
md5:3ffe0cbcf800c55e94142de79535de87
13.1 MB Download

Technical info

Tilt Series Date: 2015-07-21

Data Taken By: Debnath Ghosal

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 121.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.

Purification / Growth Conditions / Treatment: Grow culture in AB medium (pH 7) at 30C. For induction, use 375 µl of a decent ON culture to inoculate 5 ml AB mes pH 5.8 medium containing 100 µM Acetosyringone and antibiotic (Kan 50). Grow at 20C for 18 hr. Freeze cells.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-07-21-26/rawdata/

1408_atlas_20014.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB Download

1408_atlas_20014_part121_23.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB Download

Additional details

Identifiers Funding Dates
Created:
June 2, 2023
Modified:
August 31, 2025