Published 2017
| Version v1
Dataset
Open
Xanthomonas vs Caulobac (-T1SS)
Description
Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter (delta-cdzAB) competition. Caulobacter strain is a T1SS deletion strain.
Files
keyimg_dga2017-10-31-99.jpg
Technical info
Tilt Series Date: 2017-10-31
Data Taken By: Debnath Ghosal Species / Specimen: Unspecified Strain: None Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x. Acquisition Software: UCSFTomo Upload Method: reupload_downloaded Processing Software Used: TOMO3D Collaborators and Roles: Chuck Farah lab made the Xac strain, Caulobacter strain is from Laub lab. Data collected and processed by Mohammed Kaplan and Debnath Ghosal Purification / Growth Conditions / Treatment: 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing. Sample Preparation: Manual blotting, humidity 100%. 10 nm gold in 5% BSA. 4 ul Gold was applied onto the grid and blotted manually.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-10-31-99/rawdata/
Xan_CaulDelT1_20172710m0014.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download Xan_CaulDelT1_20172710m0014_SIRT_1k.rec, Reconstruction (Pixel Size 0.0 nm), 1.1 GB Download Xan_CaulDelT1_20172710m0014.ali, 377.7 MB Download Xan_CaulDelT1_20172710m0014.tlt, 424.0 B Download Xan_CaulDelT1_20172710m0014_fid.tlt, 424.0 B DownloadAdditional details
- DOI
- 10.22002/aeb8z-0tp16
- TILT_SERIES_ID
- dga2017-10-31-99
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2017-10-31
- Created
-
2017-11-01
- Updated
-
2017-11-01