Published 2017
| Version v1
Dataset
Open
U2OS-Tubulin Tracker Green
Description
U2OS cells stained with tubulin tracker to locate midbodies. Cryo-FIB milling is used to make the sample thinner.
Files
keyimg_wza2017-05-18-4.jpg
Technical info
Tilt Series Date: 2017-05-18
Data Taken By: Wei Zhao Species / Specimen: U2OS Strain: Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: SerialEM Upload Method: reupload_downloaded Processing Software Used: IMOD-patch tracking Purification / Growth Conditions / Treatment: U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL. Sample Preparation: Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wza2017-05-18-4/rawdata/
TS5.mrc, Tilt Series (Pixel Size 0.371 nm), 1.9 GB Download TS5_bin2_full.rec, Reconstruction (Pixel Size 0.0 nm), 184.9 MB Download TS5_bin4_sirt6_flipint.rec, Reconstruction (Pixel Size 0.0 nm), 356.4 MB Download TS5_bin4_sirt6_flipint.rec, Reconstruction (Pixel Size 0.0 nm), 356.4 MB Download zap000.png, 853.8 kB Download wza2017-05-18-4_zap18440.png, Periphery region of U2OS, 850.2 kB Download
Additional details
- DOI
- 10.22002/afrva-4jh12
- TILT_SERIES_ID
- wza2017-05-18-4
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2017-05-18
- Created
-
2017-07-06
- Updated
-
2017-09-14