H.pylori 26695 WT
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-01
Data Taken By: Yi-Wei Chang
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: H.pylori cells from Carrie Shaffer
Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20140301_YWC089_10009.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
20140301_YWC089_10009.rec, Reconstruction (Pixel Size 1.92 nm), 1.4 GB
Files
keyimg_ycw2014-03-01-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/ag37h-acs08
- TILT_SERIES_ID
- ycw2014-03-01-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-01
- Created
-
2014-03-01
- Updated
-
2014-03-10