E.coli "strain 2" P221D
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-02-21
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: John S. Parkinson
Purification / Growth Conditions / Treatment: cells grown at 30 degree C in TB/gentamycin to OD600: 0.38.culture was concentrated (x4) and resuspended in TB/gentamycin penicillin. Incubation 1 hour at 30 degrees.
Sample Preparation: frozen with Vitrobot settings:-4 offset3.5s blot timeno drain 3 µl sample100% humiditypure ethane
Attached Files
strain2day10019.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
strain2day10019_part.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2012-02-21-16.jpg
Additional details
Identifiers
- DOI
- 10.22002/ag6kt-12g25
- TILT_SERIES_ID
- aba2012-02-21-16
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-02-21
- Created
-
2012-02-22
- Updated
-
2012-02-23