Hippocampal Neurons (cultured)
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2014-10-18
Data Taken By: Matt Swulius
Species / Specimen: Rat Hippocampal Neuron (cultured)
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)
Purification / Growth Conditions / Treatment: Plunge frozen at DIV 14 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.
Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.
Attached Files
101814_hipp_neuron_27kx0020.mrc, Tilt Series (Pixel Size 0.41 nm), 3.4 GB
101814_hipp_neuron_27kx0020_full.rec, Reconstruction (Pixel Size 1.64 nm), 534.5 MB
Files
keyimg_mst2014-10-18-19.jpg
Additional details
Identifiers
- DOI
- 10.22002/agm82-7xj97
- TILT_SERIES_ID
- mst2014-10-18-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-10-18
- Created
-
2014-10-19
- Updated
-
2014-12-08