Bdellovibrio attack-phase

Description

Technical info

Tilt Series Date: 2012-11-14

Data Taken By: Yi-Wei Chang

Species / Specimen: Bdellovibrio bacteriovorus

Strain: HD100

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 115.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Strain from Liz Sockett

Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.

ycw2012-11-14-25_slicer14273.jpg: Bdellovibrio bacteriovorus . pink: pili . green: ribosome exclusion zone (REZ) . blue: ribosome . orange: flagllar motor & flagellum

Attached Files

Bdellovibrio_wt_20121114_0010010.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB

Bdellovibrio_wt_20121114_0010010_full.rec, Reconstruction (Pixel Size 1.6 nm), 441.7 MB