Published 2007 | Version v1
Dataset Open

Rhodopseudomonas palustrus

Creators

  • 1. ROR icon California Institute of Technology

Description

Fe-metabolizing bacterium that makes parallel membrane sheets for photosynthesis. Membrane topology is the primary interest.Keywords: photosynthetic membrane sheets, parallel membranes

Technical info

Tilt Series Date: 2007-10-04

Data Taken By: Bill Tivol

Species / Specimen: Rhodopseudomonas palustris

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1 deg°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: Diane Newman, PI, Lars Dietrich, Postdoc, Lars gave me the specimens

Purification / Growth Conditions / Treatment: Grown in FEM medium +10% acetate at 30 deg C in bright light.

Sample Preparation: quantifoil, 10 nm gold, 100% humidity

connection1tomo7.jpg: 1st connection between membrane sacks, 1st view connection1atomo7.jpg: 1st connection between membrane sacks, 2nd view connection2tomo7.jpg: 2nd connection between membrane sacks connection4tomo7.jpg: 4th connection between membrane sacks connection5tomo7.jpg: 5th connection between membrane sacks cytoinvag1tomo7.jpg: invagination of plasma membrane, 1st view cytoinvag1atomo7.jpg: invagination of plasma membrane, 2nd view btf2007-10-04-2_slicer5843.jpg: Flagella

Attached Files

Rp4Oct07_07.mrc, Tilt Series (Pixel Size 0.625 nm), 1.1 GB

Rp4Oct07_07_full.rec, Reconstruction (Pixel Size 1.25 nm), 1.2 GB

Rp4Oct07_07.rec, Reconstruction (Pixel Size 1.25 nm), 133.8 MB

Rp3Oct07_07fil.rec, Reconstruction (Pixel Size 1.25 nm) median filtered, 133.8 MB

Files

btf2007-10-04-2_slicer5843.jpg

Files (2.6 GB)

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md5:4dab49c586e21ecda3cb45da04d61124
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md5:5ef82e8fcbeb4b16fa91852d32995927
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md5:fb333342a94caff5b2c525d96849b310
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19.5 kB Preview Download
md5:745f5f479cb64ddb569eaed8ed0d8c3f
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968.9 kB Preview Download
md5:9cac2a5961b73599f7a4978fe9035e9e
18.4 MB Preview Download
133.8 MB (external) Download
1.1 GB (external) Download
133.8 MB (external) Download
1.2 GB (external) Download

Additional details

Identifiers

DOI
10.22002/ak9a4-42610
TILT_SERIES_ID
btf2007-10-04-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2007-10-04
Created
2008-06-20
Updated
2014-07-10