E. coli CusCFBA-CusC-K98H

Description

Technical info

Tilt Series Date: 2013-06-07

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain from Edward Yu

Purification / Growth Conditions / Treatment: Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.

ycw2013-06-07-7_slicer7864.jpg: Ring . Escherichia coli ycw2013-06-07-7_slicer7865.jpg: filament . Escherichia coli ycw2013-06-07-7_slicer7869.jpg: filament . Escherichia coli ycw2013-06-07-7_slicer7873.jpg: Unknown filament . DNA Toroid? . Escherichia coli ycw2013-06-07-7_slicer7879.jpg: pili . Escherichia coli . 10.2964 nm ycw2013-06-07-7_slicer8005.jpg: pili . Escherichia coli . 10 nm

Attached Files

CusCFBA_CusCK98H_20004.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

CusCFBA_CusCK98H_20004_part121_9.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB