HeLa pNL4GFP+VPS4DN
Description
Technical info
Tilt Series Date: 2011-02-10
Data Taken By: Cora Woodward
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: VPS4DN obstruct provided by Wes Sundquist. pNL4GFP construct from Irvin Chen at UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected
Sample Preparation: manual blotting
Attached Files
11feb10a__027.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB
11feb10a__027_full.rec, Reconstruction (Pixel Size 2.0 nm), 3.3 GB
Files
keyimg_cwl2011-02-10-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/aq6tq-8qj59
- TILT_SERIES_ID
- cwl2011-02-10-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-02-10
- Created
-
2015-09-16
- Updated
-
2015-09-16