Published 2015 | Version v1
Dataset Open

nanowire+doublepili mutant+Chemo

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This sample is batch 2 collection. This is from double pili mutant run on a chemostat.Batch 1 collection was from WT grown in serum bottle

Technical info

Tilt Series Date: 2015-04-11

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -4.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh, USC

Sample Preparation: Went to USC and froze using manual vitrobot. Only 1 grid was good among 6 and cells were isolated so couldn't detect any nanowires so far

Attached Files

4_atlas_nanowires0002.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

4_atlas_nanowires0002_part121_3.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_psa2015-04-11-7.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:2700dc6d04a1b09be2773f551a395411
1.1 MB Preview Download
md5:6e18985a7d2169d7b7bc5c98b0ad5d8f
17.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/argp0-eeb24
TILT_SERIES_ID
psa2015-04-11-7

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-04-11
Created
2015-04-11
Updated
2015-04-29