HELA cells expressing TRIM5a-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-02-10
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Tom Hope
Purification / Growth Conditions / Treatment: HELA cells grown for 1 day on Gold finder London EM grids, with 1/500 red beads, 41000x defocus 6.
Sample Preparation: BSL-2 hood, Vitrobot with manual blotting.
Attached Files
trimhela225000027.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
trimhela225000027_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
Files
keyimg_scd2013-02-10-27.jpg
Additional details
Identifiers
- DOI
- 10.22002/aw7xb-p6b96
- TILT_SERIES_ID
- scd2013-02-10-27
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-02-10
- Created
-
2014-02-11
- Updated
-
2015-05-07