Published 2020 | Version v1
Dataset Open

RalF-Ub V76

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Lp02 pCyaA::RalF-Ub V76 free from U937

Technical info

Tilt Series Date: 2020-07-31

Data Taken By: Yuxi Liu

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Stephen Carter

Purification / Growth Conditions / Treatment: 1h infection, 1-2 washes, fibronectin/vitronectin/no matrix. Legionella: 2.5 O.D, 3h after 200mM IPTG induction, at O.D. 3

Sample Preparation: 200mesh Gold quantifoil, 10nm gold, 37deg C, 80-90% humidity

Attached Files

RalFUbV76_180.mrc, Tilt Series (Pixel Size 0.265 nm), 1.7 GB

RalFUbV76_180_full.rec, Reconstruction (Pixel Size 1.06 nm), 516.1 MB

Files

keyimg_ylx2020-07-31-46.jpg

Files (2.3 GB)

Name Size
md5:dd25d82c8bc79de88c14235fa9ae7bf9
1.7 MB Preview Download
md5:7d9b8091e8870e7b368c2443e7351374
5.8 MB Preview Download
1.7 GB (external) Download
516.1 MB (external) Download

Additional details

Identifiers

DOI
10.22002/b1dyz-42y62
TILT_SERIES_ID
ylx2020-07-31-46

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2020-07-31
Created
2020-08-12
Updated
2021-06-30