IST1N tubes

Description

Technical info

Tilt Series Date: 2009-11-11

Data Taken By: Megan Dobro

Species / Specimen: IST1N tubes

Strain:

Tilt Series Settings: Single Axis, tilt range: (60.0°, 60.0°), step: 2 degrees°, constant angular increment, dosage: 1.0 eV/Ų, defocus: 3.5 μm, magnification: 41000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: Raptor

Collaborators and Roles: purified by John McCullough in Wes Sundquist's group

Purification / Growth Conditions / Treatment: received protein from John and performed dialysis step to crash out protein in a lower salt buffer

Sample Preparation: quantifoil, 10 nm gold, Vitrobot, 100 % humidity, 22 degrees, varying blot time and offset

Attached Files

09nov11b_Box380grid2_002_full.rec, Reconstruction (Pixel Size 0.51 nm), 187.1 MB

09nov11b_Box380grid2_002.st, Tilt Series (Pixel Size 0.51 nm), 520.1 MB