Myxococcus xanthus PilP-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-08-1_slicer8397.jpg: Myxococcus Xanthus . filament ycw2014-03-08-1_slicer8398.jpg: Myxococcus Xanthus . vesicle ycw2014-03-08-1_slicer8399.jpg: Myxococcus Xanthus . extracellular vesicle
Attached Files
20140308_YWC093_10036.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
20140308_YWC093_10036_part131_38.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2014-03-08-1.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:d04c2a5cbe5a71546c6f31a0f7c2976c
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1.0 MB | Preview Download |
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md5:6d0c1a88a9db84d72b361e27f56b3d1a
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22.4 MB | Preview Download |
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md5:ca973abf85bd445ef46d244b22a0ea6c
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432.2 kB | Preview Download |
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md5:616058fcb6caa67c73332a1cf6dc792c
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433.7 kB | Preview Download |
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md5:bd0a160f3b37916fa1de10fdbb29b3c9
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388.3 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/b3eeh-mmh68
- TILT_SERIES_ID
- ycw2014-03-08-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-08
- Created
-
2014-03-11
- Updated
-
2014-07-25