Caltech Tomography Archive
Published 2010 | Version v1
Dataset Open

Skinny E. coli with GFP-MreB

Li, Zhuo1
  • 1. ROR icon California Institute of Technology

Description

Raw data files of Skinny E. coli with GFP-MreB

Files

10mar05a_ZL001_022_prj.jpg
Files (15.6 MB)
Name Size
10mar05a_ZL001_022_prj.jpg
md5:17c9c98b8a4bbb75d26c71deb66f7b04
253.0 kB Preview Download
keymov_zla2010-03-05-22.mp4
md5:b7a43611733a7968cdc7d6fdd9a11c23
13.8 MB Download
zla2010-03-05-22_slicer9539.jpg
md5:cc57e09b071d57f4b1586c135f5a624a
371.3 kB Preview Download
keyimg_zla2010-03-05-22.jpg
md5:0f49fdeaed8a567cd45190bdd53a6ee6
1.2 MB Preview Download

Technical info

Tilt Series Date: 2010-03-05

Data Taken By: Zhuo Li

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.

Purification / Growth Conditions / Treatment: L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)

Sample Preparation: R2/2 CF or Quantifoil, 10 nm gold, 100% humidity

zla2010-03-05-22_slicer9539.jpg: Weird rainbow cell

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-22/rawdata/

10mar05a_ZL001_022.mrc, Tilt Series (Pixel Size 1.192 nm), 721.4 MB Download

10mar05a_ZL001_022_full.rec, Reconstruction (Pixel Size 2.384 nm), 524.3 MB Download

Additional details

Identifiers Funding Dates
Created:
May 18, 2023
Modified:
August 29, 2025