Published 2017 | Version v1
Dataset Open

Staphylococcus aureus_WT

  • 1. ROR icon California Institute of Technology

Description

T7SS

Technical info

Tilt Series Date: 2017-06-28

Data Taken By: Debnath Ghosal

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal.

Sample Preparation: Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot

Attached Files

Sau_WT0024.mrc, Tilt Series (Pixel Size 0.39 nm), 1.9 GB

Sau_WT0024_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

dga2017-06-28-25_slicer18620.png, , 862.9 kB

Files

dga2017-06-28-25_slicer18620.png

This file cannot be previewed
  • This file is an external reference and not stored directly in this repository. To access its content, please download it and open it locally.

Files (2.4 GB)

Name Size
862.9 kB (external) Download
md5:015e95d1cc9b2b760923016af8ddda84
1.0 MB Preview Download
md5:8a1335636ce2426cce63c000a9e2851e
13.2 MB Preview Download
1.9 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/bd6t2-pnk03
TILT_SERIES_ID
dga2017-06-28-25

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-06-28
Created
2017-06-30
Updated
2017-07-20