Published 2010 | Version v1
Dataset Open

MamJ deletion w/o iron

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

No magnetosome vesicles, suspected as a mutant that has lost the magnetosome island

Technical info

Tilt Series Date: 2010-01-22

Data Taken By: Zhuo Li

Species / Specimen: Magnetospirillum magneticum

Strain: AMB-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -16.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: Raptor and Peach, IMOD

Collaborators and Roles: Mutant constructed by Arash Komeili, cells shipped to and imaged by Zhuo Li

Purification / Growth Conditions / Treatment: Stationary phase

Sample Preparation: Quantifoid grid, 10-nm gold, 100% humidity

Attached Files

10jan22a_dJnofe2_004.mrc, Tilt Series (Pixel Size 1.192 nm), 1.0 GB

10jan22a_dJnofe2_004_part.rec, Reconstruction (Pixel Size 1.192 nm), 524.3 MB

10jan22a_dJnofe2_0041K.rec, Reconstruction (Pixel Size 1.192 nm), 271.4 MB

Files

keyimg_zla2010-01-22-1.jpg

Files (1.8 GB)

Name Size
1.0 GB (external) Download
271.4 MB (external) Download
524.3 MB (external) Download
md5:8b6351fbeb2f8f1e145ced7603f0b884
775.1 kB Preview Download
md5:bfeb523068b4e7e271b83e5d814cd7c4
9.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/bf1rg-bcq97
TILT_SERIES_ID
zla2010-01-22-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2010-01-22
Created
2010-02-12
Updated
2010-02-12