V.cholerae
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-01-06
Data Taken By: Poorna Subramanian
Species / Specimen: Vibrio cholerae
Strain: O1 biovar El Tor
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Yi-wei
Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.
psa2017-01-06-19_slicer17797.jpg: memebrane sheath on flagella is short psa2017-01-06-19_slicer17798.jpg: basal body w/o flagellum, next to this there is a basal body with flagellum
Attached Files
atlas30019_part121_4.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
atlas30019.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
Files
keyimg_psa2017-01-06-19.jpg
Files
(4.2 GB)
| Name | Size | |
|---|---|---|
| 3.4 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:e1527b53bc6b01b9e7f854f6dd1ec02c
|
1.1 MB | Preview Download |
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md5:ddb89698b8b56df50a862029cbd149ae
|
24.0 MB | Preview Download |
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md5:c5dd84e0cd7b0ef19bea24f85d468e10
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384.1 kB | Preview Download |
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md5:a3b7df8cab67961ef5f17ad4b4ad7d91
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366.8 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/bn4rx-ye708
- TILT_SERIES_ID
- psa2017-01-06-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-01-06
- Created
-
2017-01-07
- Updated
-
2017-04-05