Published 2013
| Version v1
Dataset
Open
Vibrio cholerae MKW1383
Description
In the mixture with Bdellovibrio for 4 hr at 30C. Cells seemed lost PG layer without Bdellovibrio inside.
Files
keyimg_ycw2013-10-09-40.jpg
Technical info
Tilt Series Date: 2013-10-09
Data Taken By: Yi-Wei Chang Species / Specimen: Vibrio cholerae Strain: 2740-80 Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x. Microscope: Janelia Titan Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Cells prepared by Lee Rettberg Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 4 hr at 30 C before freezing Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid ycw2013-10-09-40_slicer9848.jpg: Vibrio cholerae . DNA bundles?External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-10-09-40/rawdata/
montage_9x90013.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB Download montage_9x90013_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB DownloadAdditional details
- DOI
- 10.22002/bs1cb-c2p48
- TILT_SERIES_ID
- ycw2013-10-09-40
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2013-10-09
- Created
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2013-10-10
- Updated
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2014-08-08