JV9298
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2019-10-14
Data Taken By: Yuxi Liu
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -5.0 μm, magnification: 33000x.
Microscope: PNCC Krios 3
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Joe Vogel
Purification / Growth Conditions / Treatment: Late exponential/Early stationary
Sample Preparation: Quantifoil R2/2 copper. Vitrobot 22 deg C, 100% humidity, 4uL of 2X concentrated culture, blot force 6, blot time 3 sec, drain time 1 sec, wait time 0 sec.
Attached Files
JV9298_78_A4.mrc, Tilt Series (Pixel Size 0.1323 nm), 14.0 GB
JV9298_78_A4_full.rec, Reconstruction (Pixel Size 1.0584 nm), 737.3 MB
Files
keyimg_ylx2019-10-14-133.jpg
Additional details
Identifiers
- DOI
- 10.22002/bte2j-1x229
- TILT_SERIES_ID
- ylx2019-10-14-133
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-10-14
- Created
-
2019-12-17
- Updated
-
2020-01-02