Coxiella_ Verocells_28dpi
Description
Technical info
Tilt Series Date: 2017-10-19
Data Taken By: Debnath Ghosal
Species / Specimen: Coxiella burnetii
Strain: Nine Mile phase II clone 4
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal and Mohammed Kaplan using Caltech tomography pipeline.
Purification / Growth Conditions / Treatment: Refer to Heinzen lab.
Sample Preparation: Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.
Attached Files
Cb_vero_201710190037.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB
Cb_vero_201710190037_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB
Files
keyimg_dga2017-10-19-39.jpg
Additional details
Identifiers
- DOI
- 10.22002/bwa0s-s3484
- TILT_SERIES_ID
- dga2017-10-19-39
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-10-19
- Created
-
2017-10-23
- Updated
-
2017-10-24