H.pylori 26695 WT/AGS

Description

Technical info

Tilt Series Date: 2014-02-14

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 205.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori & AGS cells from Carrie Shaffer

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.

ycw2014-02-14-1_slicer3639.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Tube, possibly T4SS related projecting from Hp cell. ycw2014-02-14-1_slicer3640.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Tube, possibly T4SS related projecting from Hp cell. ycw2014-02-14-1_slicer3641.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Tube, possibly T4SS related projecting from Hp cell. ycw2014-02-14-1_slicer3642.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Tube, possibly T4SS related projecting from Hp cell. Tube has bend in it. ycw2014-02-14-1_slicer3643.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Tube, possibly T4SS related projecting from Hp cell. Tube has bend in it. ycw2014-02-14-1_slicer3644.jpg: Helicobacter pylori 26695 WT co-cultured with AGS cells. . . Curvy filament bundles run along inside of inner membrane (bottom arrow). Parainfluenza virus particles from AGS cells(top arrow)

Attached Files

20140214_YWC081_10001.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

20140214_YWC081_10001_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB