Published 2010 | Version v1
Dataset Open

MreB o/e 8hr single colony

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

Raw data files of MreB o/e 8hr single colony

Technical info

Tilt Series Date: 2010-01-09

Data Taken By: Zhuo Li

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Cells cultured and imaged by ZL

Purification / Growth Conditions / Treatment: Single colony was picked from a PYE plate w/ 0.2 glucose and cultured o/n. The next day cells were diluted to exponential phase and induced for 8hrs w/ 0.3% xylose.

Sample Preparation: R2/2 or R2/1, 10nm gold, 100% humidity

Attached Files

10jan09a_MreBoe_037.mrc, Tilt Series (Pixel Size 1.192 nm), 1.1 GB

10jan09a_MreBoe_037_part.rec, Reconstruction (Pixel Size 2.384 nm), 524.3 MB

Files

keyimg_zla2010-01-09-37.jpg

Files (1.6 GB)

Name Size
1.1 GB (external) Download
524.3 MB (external) Download
md5:d95fe5ac84e91684771470b19b612b02
1.1 MB Preview Download
md5:ba8befb7d0ecc2a83457b2217f4bf06a
15.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/bydb3-fnt90
TILT_SERIES_ID
zla2010-01-09-37

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2010-01-09
Created
2010-03-24
Updated
2010-03-25