Published 2009 | Version v1
Dataset Open

Hyphomonas EB session21 34KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-07-14

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: raptor

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in PYE media, EB 0.02mg/ml overnight 4degree...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

jsa2009-07-14-52_slicer8898.jpg: Hyphomonas Neptunium . DNA/ inclusion body

Attached Files

09jul14a_b200c_052.mrc, Tilt Series (Pixel Size 0.632 nm), 536.9 MB

09jul14a_b200c_052_full.rec, Reconstruction (Pixel Size 1.264 nm), 367.0 MB

Files

jsa2009-07-14-52_slicer8898.jpg

Files (915.9 MB)

Name Size
536.9 MB (external) Download
367.0 MB (external) Download
md5:5f49e633cad38f1ddf741f30767c348f
384.3 kB Preview Download
md5:e1f20dc65d2ae50eafc12f596f396926
1.1 MB Preview Download
md5:9759ed356d72897887c7a29131d2cd49
10.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/bz45v-5z430
TILT_SERIES_ID
jsa2009-07-14-52

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-07-14
Created
2009-10-20
Updated
2014-07-29