Published 2013
| Version v1
Dataset
Open
B subtilis bMD204
Description
mreB-40aa-sfGFP sfGFP-11aa-mbl::spec ΔponA::cat"dual-tagged" strain, where mreB is C-terminally tagged and includes a 40 aa linker, while mbl is N-terminally tagged and uses an 11 aa linker.
Technical info
Tilt Series Date: 2013-11-15
Data Taken By: Matt Swulius
Species / Specimen: Bacillus subtilis
Strain: bMD204
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: raptor
Collaborators and Roles: Ethan Garner and Mike Dion
Attached Files
111510_bs2040013.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB
Files
keyimg_mst2013-11-15-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/c059s-6jf34
- TILT_SERIES_ID
- mst2013-11-15-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-11-15
- Created
-
2013-12-03
- Updated
-
2014-03-31