nanochemowt
Description
Technical info
Tilt Series Date: 2015-06-02
Data Taken By: Poorna Subramanian
Species / Specimen: Shewanella oneidensis
Strain: MR-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Moh and Sahand USC
Sample Preparation: This sample was frozen after being in oxygen deficiency for 16h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.
psa2015-06-02-24_slicer13887.jpg: sheathed flagellum or nanowire? psa2015-06-02-24_slicer13888.jpg: sheathed flagellum or nanowire? psa2015-06-02-24_slicer13889.jpg: sheathed flagellum or nanowire?
Attached Files
nanowire_manual4.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
nanowire_manual4_part121_7.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB
Files
keyimg_psa2015-06-02-24.jpg
Files
(4.0 GB)
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md5:fc612919696669348b5d32cd694138b0
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md5:b8e3abf9ce2e11e3ed57bffcb7ad3d88
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19.1 MB | Preview Download |
| 3.4 GB | (external) Download | |
| 534.5 MB | (external) Download | |
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md5:038848bc0c6458507cff3f16b5da71c2
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398.7 kB | Preview Download |
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md5:8a743944597716c5d0b44dbfa322d983
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398.8 kB | Preview Download |
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md5:81431912265c6dc2efbf25f96014b488
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267.9 kB | Preview Download |
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md5:8a743944597716c5d0b44dbfa322d983
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398.8 kB | Preview Download |
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md5:7987fb747c13db9cc81c7da78db44b1d
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351.2 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/c4rxs-4kj96
- TILT_SERIES_ID
- psa2015-06-02-24
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-06-02
- Created
-
2015-06-02
- Updated
-
2015-06-07