Myxococcus xanthus TM530
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-10-25
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Tam Mignot
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200
ycw2013-10-25-42_slicer9092.jpg: Myxococcus Xanthus . OMV . pili ycw2013-10-25-42_slicer9094.jpg: Myxococcus Xanthus . Plate ycw2013-10-25-42_slicer9097.jpg: Myxococcus Xanthus . filament bundle
Attached Files
20131025_YWC029_10012.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
20131025_YWC029_10012_part121_1.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2013-10-25-42.jpg
Files
(4.2 GB)
| Name | Size | |
|---|---|---|
| 3.4 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:56fce1492f6a3bad55a41fc3228aaad7
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1.1 MB | Preview Download |
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md5:d3b8a45c46d01bf149c8ef00be9cd465
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22.4 MB | Preview Download |
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md5:b94b364f1cce207e8a3343ca8487f49d
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250.0 kB | Preview Download |
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md5:50df29f24f841cbfa698e82479adfca3
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303.9 kB | Preview Download |
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md5:be8e165265c30a4adafaf55d4424368d
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405.4 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/c4s2n-by758
- TILT_SERIES_ID
- ycw2013-10-25-42
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-10-25
- Created
-
2013-10-26
- Updated
-
2014-07-31