Published 2017
| Version v1
Dataset
Open
E. cloacae minicells/locked T6SS
Contributors
Researcher:
Description
Grid box YWC253E. cloacae cells overexpressing MinE for 40min to produce fresh minicellsStrain ECL296: TssC E341I (to potentially lock T6SS sheath in the extended state)
Technical info
Tilt Series Date: 2017-06-05
Data Taken By: Yi-Wei Chang
Species / Specimen: Enterobacter cloacae
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Zachary Ruhe
ycw2017-06-05-16_slicer18127.jpg: contracted T6SS
Attached Files
20170605_YWC253_10024.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
20170605_YWC253_10024_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB
Files
keyimg_ycw2017-06-05-16.jpg
Additional details
Identifiers
- DOI
- 10.22002/c4sk9-3s028
- TILT_SERIES_ID
- ycw2017-06-05-16
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-06-05
- Created
-
2017-06-05
- Updated
-
2017-06-13