Published 2011 | Version v1
Dataset Open

E.coli "strain8" Tsr-A413G

  • 1. ROR icon California Institute of Technology

Description

E. coli methylation site mutant, locked in the CheA activating state

Technical info

Tilt Series Date: 2011-10-14

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-70.0°, 70.0°), step: °, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: webload

Processing Software Used:

Collaborators and Roles: mutant from John S. Parkinson. Data collected on the UCLA TITAN

Purification / Growth Conditions / Treatment: cells grown at 30C to log phase, then lysed with penicillin for one hour

Attached Files

ecoli-tomo_22.st, Tilt Series (Pixel Size 0.0 nm), 1.2 GB

ecoli-tomo_22.rec, Reconstruction (Pixel Size 0.0 nm), 1.5 GB

Files

keyimg_aba2011-10-14-18.jpg

Files (2.7 GB)

Name Size
1.5 GB (external) Download
1.2 GB (external) Download
md5:ba7eaa5ed5e32a11d6a77215db4cea12
4.7 MB Preview Download
md5:a83f3968f9863c97fabccd5e4a016e0e
9.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/cbg2s-rtw70
TILT_SERIES_ID
aba2011-10-14-18

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-10-14
Created
2011-10-24
Updated
2011-10-24