Caltech Tomography Archive
Published 2012 | Version v1
Dataset Open

COS7 VPS4DN+pNLGFP

Woodward, Cora1
  • 1. ROR icon California Institute of Technology

Description

Cos7 cells transfected with VPS4DN (0.5 ug) and pNLGFP provirus construct (4 ug).

Files

keyimg_cwl2012-11-29-12.jpg
Files (21.2 MB)
Name Size
keymov_cwl2012-11-29-12.mp4
md5:32eeecd01b564e13d779eb2fdd66f189
20.1 MB Download
keyimg_cwl2012-11-29-12.jpg
md5:76bb7de320b426d1d9b1f53aaedab6f9
1.1 MB Preview Download

Technical info

Tilt Series Date: 2012-11-29

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine

Sample Preparation: manual blotting

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-29-12/rawdata/

12nov29_C129_1_pnlDN_AT2BT1_0005.mrc, Tilt Series (Pixel Size 0.49 nm), 3.4 GB Download

12nov29_C129_1_pnlDN_AT2BT1_0005_part122_15.rec, Reconstruction (Pixel Size 1.96 nm), 530.0 MB Download

Additional details

Identifiers Funding Dates
Created:
May 20, 2023
Modified:
August 27, 2025