Published 2020
| Version v1
Dataset
Open
P. gingivalis wild type
Description
Type IX secretion system, t9ss
Files
keyimg_gca2020-10-16-118.jpg
Technical info
Tilt Series Date: 2020-10-16
Data Taken By: Georges Chreifi Species / Specimen: Porphyromonas gingivalis Strain: Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x. Microscope: Caltech Titan Krios Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Eric Reynolds Purification / Growth Conditions / Treatment: Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days. Sample Preparation: Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE method.External Files
Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-118
PginWT_8_116.mrc, Tilt Series (Pixel Size 0.333 nm), 1.9 GB Download PginWT_8_116_full.rec, Reconstruction (Pixel Size 1.332 nm), 884.7 MB DownloadAdditional details
- TILT_SERIES_ID
- gca2020-10-16-118
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
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2020-10-16
- Created
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2020-10-18
- Updated
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2021-08-24